Fig. 10. NTP induces p53 dependant apoptosis in Huh7 cells. (A) NTP treatment induces Bcl-2 upregulation in HepG2 and downregulation of p53 in Huh7 cells. Cells were treated with NTPs for 60 s. Cells were analysed by Western immunoblotting 4 h after treatment. Actin - control of equal protein loading. The graphs show densitometric quantification of p53 and Bcl-2 Western immunoblots, *P< 0.05, **P< 0.01, ***P< 0.001, mean ± SEM, n = 3. Representative blots out of three independent experiments are shown. Non-irradiated cells treated with H₂O₂ (10 mM) were used as a positive control (full blots are presented in Appendix A). (B) NTP treatment downregulates phosphorylation of Stat1 in Huh7 cells. Cells were treated as in (A) and analysed by Western immunoblotting 4 h after treatment. Actin - control of equal protein loading (full blots are presented in Appendix A). (C) Bcl-2 and p53were analysed in whole cell lysates of HepG2, Huh7, Alexander cells, and human liver by immunoblotting; Actin - control of equal protein loading (full blots are presented in Appendix A).